Primers Available
Organism: Cucumaria miniata mitochondrion
Repeat:(AATA)3
Start:10546 End:10557
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAACCGGTCAGCAGAGAGTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCTGCTTTGGGTTTGGTTC | |||||||||||||
| Tm_F_1 | 60.389 | Size_F_1 | 20 | Tm_R_1 | 59.536 | Size_R_1 | 20 | PRODUCT Size | 154 bp | Primer Start | 10519 | Primer End | 10672 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAACCGGTCAGCAGAGAGTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCGGAATAGGGGGTGTCTCT | |||||||||||||
| Tm_F_2 | 60.389 | Size_F_2 | 20 | Tm_R_2 | 59.365 | Size_R_2 | 20 | PRODUCT Size | 275 bp | Primer Start | 10519 | Primer End | 10793 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAACCGGTCAGCAGAGAGTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCTGCTTTGGGTTTGGTTCT | |||||||||||||
| Tm_F_3 | 60.389 | Size_F_3 | 20 | Tm_R_3 | 59.247 | Size_R_3 | 20 | PRODUCT Size | 153 bp | Primer Start | 10519 | Primer End | 10671 |