Primers Available
Organism: Saccharum hybrid cultivar SP-80-3280 chloroplast
Repeat:(TATAA)3
Start:82647 End:82661
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGTGGATTTCCAGTCGCAGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AATGCTGTTCGGACCTGGAG | |||||||||||||
| Tm_F_1 | 60.036 | Size_F_1 | 20 | Tm_R_1 | 60.036 | Size_R_1 | 20 | PRODUCT Size | 233 bp | Primer Start | 82593 | Primer End | 82825 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTCGCAGGCTGCAAAATTCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AATGCTGTTCGGACCTGGAG | |||||||||||||
| Tm_F_2 | 60.039 | Size_F_2 | 20 | Tm_R_2 | 60.036 | Size_R_2 | 20 | PRODUCT Size | 221 bp | Primer Start | 82605 | Primer End | 82825 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGCAGGCTGCAAAATTCAGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AATGCTGTTCGGACCTGGAG | |||||||||||||
| Tm_F_3 | 59.758 | Size_F_3 | 20 | Tm_R_3 | 60.036 | Size_R_3 | 20 | PRODUCT Size | 219 bp | Primer Start | 82607 | Primer End | 82825 |