Primers Available
Organism: Saccharum hybrid cultivar SP-80-3280 chloroplast
Repeat:(GTAG)4
Start:116260 End:116275
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCATACGGCGGGAGTCATTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGATACGAGTCGAGGCTGT | |||||||||||||
| Tm_F_1 | 59.896 | Size_F_1 | 20 | Tm_R_1 | 60.107 | Size_R_1 | 20 | PRODUCT Size | 198 bp | Primer Start | 116225 | Primer End | 116422 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATTGCTTTCATACGGCGGGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGGATACGAGTCGAGGCTGT | |||||||||||||
| Tm_F_2 | 60.107 | Size_F_2 | 20 | Tm_R_2 | 60.107 | Size_R_2 | 20 | PRODUCT Size | 205 bp | Primer Start | 116218 | Primer End | 116422 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTTTCATACGGCGGGAGTCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGGATACGAGTCGAGGCTGT | |||||||||||||
| Tm_F_3 | 59.825 | Size_F_3 | 20 | Tm_R_3 | 60.107 | Size_R_3 | 20 | PRODUCT Size | 201 bp | Primer Start | 116222 | Primer End | 116422 |