Primers Available
Organism: Saccharum hybrid cultivar SP-80-3280 chloroplast
Repeat:(AGAA)3
Start:132520 End:132531
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTTTTGGAACACCAATGGGCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCATTCGAATCACGGATTCCT | |||||||||||||
| Tm_F_1 | 59.584 | Size_F_1 | 21 | Tm_R_1 | 57.18 | Size_R_1 | 21 | PRODUCT Size | 256 bp | Primer Start | 132430 | Primer End | 132685 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACTTTTGGAACACCAATGGGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCATTCGAATCACGGATTCCT | |||||||||||||
| Tm_F_2 | 59.584 | Size_F_2 | 21 | Tm_R_2 | 57.18 | Size_R_2 | 21 | PRODUCT Size | 257 bp | Primer Start | 132429 | Primer End | 132685 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTTGGAACACCAATGGGCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCATTCGAATCACGGATTCCT | |||||||||||||
| Tm_F_3 | 58.5 | Size_F_3 | 20 | Tm_R_3 | 57.18 | Size_R_3 | 21 | PRODUCT Size | 255 bp | Primer Start | 132431 | Primer End | 132685 |