Primers Available
Organism: Saccharum hybrid cultivar SP-80-3280 chloroplast
Repeat:(AAAG)3
Start:67800 End:67811
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGATTGGTCGTTTTTGGGTAGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCGAAAACGATCTCGATCTGTG | |||||||||||||
| Tm_F_1 | 57.905 | Size_F_1 | 22 | Tm_R_1 | 60.035 | Size_R_1 | 22 | PRODUCT Size | 275 bp | Primer Start | 67669 | Primer End | 67943 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGAAAAGATTGGTCGTTTTTGGGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCGAAAACGATCTCGATCTGTG | |||||||||||||
| Tm_F_2 | 59.839 | Size_F_2 | 24 | Tm_R_2 | 60.035 | Size_R_2 | 22 | PRODUCT Size | 280 bp | Primer Start | 67664 | Primer End | 67943 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGATTGGTCGTTTTTGGGTAGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGCGAAAACGATCTCGATCTGT | |||||||||||||
| Tm_F_3 | 57.905 | Size_F_3 | 22 | Tm_R_3 | 60.159 | Size_R_3 | 22 | PRODUCT Size | 276 bp | Primer Start | 67669 | Primer End | 67944 |