Primers Available
Organism: Dirofilaria immitis mitochondrion
Repeat:(TTG)4
Start:5138 End:5149
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGTCAGATGAGTTATTGAGCTGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCTTCAACCAATAGCCCGGA | |||||||||||||
| Tm_F_1 | 59.441 | Size_F_1 | 23 | Tm_R_1 | 59.745 | Size_R_1 | 20 | PRODUCT Size | 277 bp | Primer Start | 4978 | Primer End | 5254 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGTCAGATGAGTTATTGAGCTGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCCTTCAACCAATAGCCCGG | |||||||||||||
| Tm_F_2 | 59.441 | Size_F_2 | 23 | Tm_R_2 | 59.745 | Size_R_2 | 20 | PRODUCT Size | 278 bp | Primer Start | 4978 | Primer End | 5255 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGAGTTATTGAGCTGCTGTTGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCTTCAACCAATAGCCCGGA | |||||||||||||
| Tm_F_3 | 58.256 | Size_F_3 | 22 | Tm_R_3 | 59.745 | Size_R_3 | 20 | PRODUCT Size | 270 bp | Primer Start | 4985 | Primer End | 5254 |