Primers Available
Organism: Dirofilaria immitis mitochondrion
Repeat:(T)13ctttgttggtgtattatgtttatttttttgaatctgttttgtttttttattttatgtgttttggggttatttctggtgtagttggtttggtta(T)12
Start:7257 End:7374
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGGTATTTCTTTTTGTGTTGACTTTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCTTATCAAAACCATACCCCTT | |||||||||||||
| Tm_F_1 | 57.281 | Size_F_1 | 27 | Tm_R_1 | 57.454 | Size_R_1 | 23 | PRODUCT Size | 226 bp | Primer Start | 7176 | Primer End | 7401 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGGTATTTCTTTTTGTGTTGACTTTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AACCTTATCAAAACCATACCCCT | |||||||||||||
| Tm_F_2 | 57.281 | Size_F_2 | 27 | Tm_R_2 | 57.454 | Size_R_2 | 23 | PRODUCT Size | 227 bp | Primer Start | 7176 | Primer End | 7402 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGGTATTTCTTTTTGTGTTGACTTTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AACCTTATCAAAACCATACCCCTT | |||||||||||||
| Tm_F_3 | 57.281 | Size_F_3 | 27 | Tm_R_3 | 58.089 | Size_R_3 | 24 | PRODUCT Size | 227 bp | Primer Start | 7176 | Primer End | 7402 |