Primers Available
Organism: Dirofilaria immitis mitochondrion
Repeat:(T)12
Start:1145 End:1156
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGGAGTGTGTGTCAGTTTGTGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCAGACACACAAATAAACCCCA | |||||||||||||
| Tm_F_1 | 60.027 | Size_F_1 | 22 | Tm_R_1 | 57.828 | Size_R_1 | 22 | PRODUCT Size | 210 bp | Primer Start | 1014 | Primer End | 1223 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGGAGTGTGTGTCAGTTTGTGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACCAACCAAAATTTCAGACACACA | |||||||||||||
| Tm_F_2 | 60.027 | Size_F_2 | 22 | Tm_R_2 | 59.778 | Size_R_2 | 24 | PRODUCT Size | 223 bp | Primer Start | 1014 | Primer End | 1236 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGAGTGTGTGTCAGTTTGTGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCAGACACACAAATAAACCCCA | |||||||||||||
| Tm_F_3 | 58.644 | Size_F_3 | 21 | Tm_R_3 | 57.828 | Size_R_3 | 22 | PRODUCT Size | 209 bp | Primer Start | 1015 | Primer End | 1223 |