Primers Available
Organism: Ixodes holocyclus mitochondrion
Repeat:(T)12
Start:976 End:987
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCCTTCCTCACTTGGAAATATGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGCAAGTTTAACGAAGAAAATTTCT | |||||||||||||
| Tm_F_1 | 59.46 | Size_F_1 | 24 | Tm_R_1 | 57.304 | Size_R_1 | 26 | PRODUCT Size | 262 bp | Primer Start | 863 | Primer End | 1124 |
| FORWARD_PRIMER_2 | |||||||||||||
| AATCCCTTCCTCACTTGGAAAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTGCAAGTTTAACGAAGAAAATTTCT | |||||||||||||
| Tm_F_2 | 57.318 | Size_F_2 | 22 | Tm_R_2 | 57.304 | Size_R_2 | 26 | PRODUCT Size | 264 bp | Primer Start | 861 | Primer End | 1124 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCTTCCTCACTTGGAAATATGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGCAAGTTTAACGAAGAAAATTTCT | |||||||||||||
| Tm_F_3 | 58.14 | Size_F_3 | 23 | Tm_R_3 | 57.304 | Size_R_3 | 26 | PRODUCT Size | 261 bp | Primer Start | 864 | Primer End | 1124 |