Primers Available
Organism: Etheostoma radiosum mitochondrion
Repeat:(CTT)4
Start:6057 End:6068
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTCCCGCCATCTCCCAATAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGTGGCCGAAGAATCAGAA | |||||||||||||
| Tm_F_1 | 59.965 | Size_F_1 | 20 | Tm_R_1 | 60.035 | Size_R_1 | 20 | PRODUCT Size | 205 bp | Primer Start | 5994 | Primer End | 6198 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCGCCATCTCCCAATACCAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGGTGGCCGAAGAATCAGAA | |||||||||||||
| Tm_F_2 | 59.817 | Size_F_2 | 20 | Tm_R_2 | 60.035 | Size_R_2 | 20 | PRODUCT Size | 202 bp | Primer Start | 5997 | Primer End | 6198 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCCAATACCAAACGCCGTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGTGGCCGAAGAATCAGAA | |||||||||||||
| Tm_F_3 | 60.251 | Size_F_3 | 20 | Tm_R_3 | 60.035 | Size_R_3 | 20 | PRODUCT Size | 194 bp | Primer Start | 6005 | Primer End | 6198 |