Primers Available
Organism: Calycanthus floridus var. glaucus chloroplast
Repeat:(TTTG)3
Start:136369 End:136380
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGGCGTTAAGAGACCCTCCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCCTAATCCTCCCTTCCCGA | |||||||||||||
| Tm_F_1 | 59.959 | Size_F_1 | 20 | Tm_R_1 | 60.105 | Size_R_1 | 20 | PRODUCT Size | 271 bp | Primer Start | 136301 | Primer End | 136571 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGGCGTTAAGAGACCCTCCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCCCTAATCCTCCCTTCCCG | |||||||||||||
| Tm_F_2 | 59.959 | Size_F_2 | 20 | Tm_R_2 | 60.105 | Size_R_2 | 20 | PRODUCT Size | 272 bp | Primer Start | 136301 | Primer End | 136572 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTACTGCGCTCTCCAAGTGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACCCCCTCAGTCACTACGAA | |||||||||||||
| Tm_F_3 | 60.038 | Size_F_3 | 20 | Tm_R_3 | 59.886 | Size_R_3 | 20 | PRODUCT Size | 242 bp | Primer Start | 136208 | Primer End | 136449 |