Primers Available
Organism: Calycanthus floridus var. glaucus chloroplast
Repeat:(T)15
Start:4449 End:4463
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATTGCCCGACCCCATCAAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CAAATGCTGGTGCTGGGTTC | |||||||||||||
| Tm_F_1 | 60.252 | Size_F_1 | 20 | Tm_R_1 | 60.038 | Size_R_1 | 20 | PRODUCT Size | 249 bp | Primer Start | 4278 | Primer End | 4526 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATTGCCCGACCCCATCAAAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGGTTCACCAGATTCGCTG | |||||||||||||
| Tm_F_2 | 60.252 | Size_F_2 | 20 | Tm_R_2 | 60.322 | Size_R_2 | 20 | PRODUCT Size | 236 bp | Primer Start | 4278 | Primer End | 4513 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATTGCCCGACCCCATCAAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGTTCACCAGATTCGCTGT | |||||||||||||
| Tm_F_3 | 60.252 | Size_F_3 | 20 | Tm_R_3 | 60.322 | Size_R_3 | 20 | PRODUCT Size | 235 bp | Primer Start | 4278 | Primer End | 4512 |