Primers Available
Organism: Calycanthus floridus var. glaucus chloroplast
Repeat:(T)13
Start:81801 End:81813
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGTGTCCCTACCCATGACGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCGATTGCTCGGGAATTGAG | |||||||||||||
| Tm_F_1 | 59.959 | Size_F_1 | 20 | Tm_R_1 | 59.694 | Size_R_1 | 20 | PRODUCT Size | 255 bp | Primer Start | 81732 | Primer End | 81986 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGGCAATAGTGTCCCTACCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCGATTGCTCGGGAATTGAG | |||||||||||||
| Tm_F_2 | 59.894 | Size_F_2 | 20 | Tm_R_2 | 59.694 | Size_R_2 | 20 | PRODUCT Size | 262 bp | Primer Start | 81725 | Primer End | 81986 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGTCCCTACCCATGACGAAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCGATTGCTCGGGAATTGAG | |||||||||||||
| Tm_F_3 | 59.1 | Size_F_3 | 20 | Tm_R_3 | 59.694 | Size_R_3 | 20 | PRODUCT Size | 253 bp | Primer Start | 81734 | Primer End | 81986 |