Primers Available
Organism: Calycanthus floridus var. glaucus chloroplast
Repeat:(T)13
Start:32166 End:32178
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGACACGACAGAAAAAGGGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGGATTCGACTCGTACCCT | |||||||||||||
| Tm_F_1 | 59.765 | Size_F_1 | 20 | Tm_R_1 | 59.742 | Size_R_1 | 20 | PRODUCT Size | 270 bp | Primer Start | 31943 | Primer End | 32212 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGACACGACAGAAAAAGGGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGCGATGAAGGGATTCGACT | |||||||||||||
| Tm_F_2 | 59.765 | Size_F_2 | 20 | Tm_R_2 | 59.176 | Size_R_2 | 20 | PRODUCT Size | 278 bp | Primer Start | 31943 | Primer End | 32220 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCGACACGACAGAAAAAGGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGGGATTCGACTCGTACCCT | |||||||||||||
| Tm_F_3 | 59.134 | Size_F_3 | 20 | Tm_R_3 | 59.742 | Size_R_3 | 20 | PRODUCT Size | 271 bp | Primer Start | 31942 | Primer End | 32212 |