Primers Available
Organism: Calycanthus floridus var. glaucus chloroplast
Repeat:(ATA)4
Start:119129 End:119140
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGAACAACAATTTCCCTGATTTGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGTGCAATTGCTATGGCTCG | |||||||||||||
| Tm_F_1 | 57.523 | Size_F_1 | 24 | Tm_R_1 | 59.97 | Size_R_1 | 20 | PRODUCT Size | 270 bp | Primer Start | 118966 | Primer End | 119235 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGAACAAGTACGTAACAAAGAAAGGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTGCAATTGCTATGGCTCG | |||||||||||||
| Tm_F_2 | 59.18 | Size_F_2 | 26 | Tm_R_2 | 59.97 | Size_R_2 | 20 | PRODUCT Size | 235 bp | Primer Start | 119001 | Primer End | 119235 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACAATTTCCCTGATTTGATTGACT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTGCAATTGCTATGGCTCG | |||||||||||||
| Tm_F_3 | 57.077 | Size_F_3 | 24 | Tm_R_3 | 59.97 | Size_R_3 | 20 | PRODUCT Size | 264 bp | Primer Start | 118972 | Primer End | 119235 |