Primers Available
Organism: Calycanthus floridus var. glaucus chloroplast
Repeat:(AT)8
Start:28497 End:28512
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGATTCTGTGGCTGGAAATCCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TATTGATCAGGCGACACCCG | |||||||||||||
| Tm_F_1 | 59.959 | Size_F_1 | 22 | Tm_R_1 | 59.896 | Size_R_1 | 20 | PRODUCT Size | 259 bp | Primer Start | 28343 | Primer End | 28601 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGATTCTGTGGCTGGAAATCCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACACCCGGATTTGAACTGGG | |||||||||||||
| Tm_F_2 | 59.959 | Size_F_2 | 22 | Tm_R_2 | 60.251 | Size_R_2 | 20 | PRODUCT Size | 246 bp | Primer Start | 28343 | Primer End | 28588 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGTTCCAATTTGCCCTGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACACCCGGATTTGAACTGGG | |||||||||||||
| Tm_F_3 | 57.906 | Size_F_3 | 20 | Tm_R_3 | 60.251 | Size_R_3 | 20 | PRODUCT Size | 270 bp | Primer Start | 28319 | Primer End | 28588 |