Primers Available
Organism: Calycanthus floridus var. glaucus chloroplast
Repeat:(AAT)4
Start:124517 End:124528
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACACAGATATGGATTTTCCCGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCTTAGGTCCAAACGTGGGT | |||||||||||||
| Tm_F_1 | 57.229 | Size_F_1 | 22 | Tm_R_1 | 58.573 | Size_R_1 | 20 | PRODUCT Size | 179 bp | Primer Start | 124489 | Primer End | 124667 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACACAGATATGGATTTTCCCGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTCTTAGGTCCAAACGTGGGT | |||||||||||||
| Tm_F_2 | 57.229 | Size_F_2 | 22 | Tm_R_2 | 59.23 | Size_R_2 | 21 | PRODUCT Size | 180 bp | Primer Start | 124489 | Primer End | 124668 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACACAGATATGGATTTTCCCGATCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCTTAGGTCCAAACGTGGGT | |||||||||||||
| Tm_F_3 | 60.163 | Size_F_3 | 25 | Tm_R_3 | 58.573 | Size_R_3 | 20 | PRODUCT Size | 179 bp | Primer Start | 124489 | Primer End | 124667 |