Primers Available
Organism: Calycanthus floridus var. glaucus chloroplast
Repeat:(A)13
Start:5397 End:5409
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTCCTCATACGGCTCGAGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGAGGTTAGAGACGGCTCAA | |||||||||||||
| Tm_F_1 | 59.531 | Size_F_1 | 20 | Tm_R_1 | 57.782 | Size_R_1 | 20 | PRODUCT Size | 220 bp | Primer Start | 5282 | Primer End | 5501 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTCCTCATACGGCTCGAGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAGAGGTTAGAGACGGCTCA | |||||||||||||
| Tm_F_2 | 59.531 | Size_F_2 | 20 | Tm_R_2 | 57.782 | Size_R_2 | 20 | PRODUCT Size | 221 bp | Primer Start | 5282 | Primer End | 5502 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTCCTCATACGGCTCGAGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAGAGGTTAGAGACGGCTCAA | |||||||||||||
| Tm_F_3 | 59.531 | Size_F_3 | 20 | Tm_R_3 | 58.474 | Size_R_3 | 21 | PRODUCT Size | 221 bp | Primer Start | 5282 | Primer End | 5502 |