Primers Available
Organism: Sphenodon punctatus mitochondrion
Repeat:(AAT)4
Start:629 End:640
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TACGCTCTCATAGGGGCCAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATGGGGCTCGGTTTGTTTCT | |||||||||||||
| Tm_F_1 | 60.179 | Size_F_1 | 20 | Tm_R_1 | 59.888 | Size_R_1 | 20 | PRODUCT Size | 214 bp | Primer Start | 467 | Primer End | 680 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATACGCTCTCATAGGGGCCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATGGGGCTCGGTTTGTTTCT | |||||||||||||
| Tm_F_2 | 60.179 | Size_F_2 | 20 | Tm_R_2 | 59.888 | Size_R_2 | 20 | PRODUCT Size | 215 bp | Primer Start | 466 | Primer End | 680 |
| FORWARD_PRIMER_3 | |||||||||||||
| TACGCTCTCATAGGGGCCAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCAAATGGGGCTCGGTTTGT | |||||||||||||
| Tm_F_3 | 60.179 | Size_F_3 | 20 | Tm_R_3 | 60.179 | Size_R_3 | 20 | PRODUCT Size | 218 bp | Primer Start | 467 | Primer End | 684 |