Primers Available
Organism:Monoblepharella sp. JEL15 mitochondrion
Repeat:(G)14
Start:31067 End:31080
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAGATTCCTCAAGTTCCCCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAACGGCCTTCCTACACCTT | |||||||||||||
| Tm_F_1 | 60.042 | Size_F_1 | 20 | Tm_R_1 | 59.998 | Size_R_1 | 20 | PRODUCT Size | 236 bp | Primer Start | 30870 | Primer End | 31105 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTATGGCCCCCTCAGGTTAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAACGGCCTTCCTACACCTT | |||||||||||||
| Tm_F_2 | 60.166 | Size_F_2 | 20 | Tm_R_2 | 59.998 | Size_R_2 | 20 | PRODUCT Size | 152 bp | Primer Start | 30954 | Primer End | 31105 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCAGGAGCCTACACCTTCAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAGGGACGGGCTTACAGATT | |||||||||||||
| Tm_F_3 | 59.861 | Size_F_3 | 20 | Tm_R_3 | 59.962 | Size_R_3 | 20 | PRODUCT Size | 231 bp | Primer Start | 31034 | Primer End | 31264 |