Primers Available
Organism: Pholis crassispina mitochondrion
Repeat:(TCC)4
Start:5805 End:5816
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCGAGCAGAGTTAAGCCAGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATACCGTCCACCCTGTACCA | |||||||||||||
| Tm_F_1 | 60.109 | Size_F_1 | 20 | Tm_R_1 | 59.958 | Size_R_1 | 20 | PRODUCT Size | 275 bp | Primer Start | 5589 | Primer End | 5863 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCAATCATGATCGGGGGCTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGCGTGCGCTAAATTACCAG | |||||||||||||
| Tm_F_2 | 60.179 | Size_F_2 | 20 | Tm_R_2 | 59.972 | Size_R_2 | 20 | PRODUCT Size | 204 bp | Primer Start | 5692 | Primer End | 5895 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCAATCATGATCGGGGGCTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATACCGTCCACCCTGTACCA | |||||||||||||
| Tm_F_3 | 60.179 | Size_F_3 | 20 | Tm_R_3 | 59.958 | Size_R_3 | 20 | PRODUCT Size | 172 bp | Primer Start | 5692 | Primer End | 5863 |