Primers Available
Organism:Cryptococcus neoformans var. grubii mitochondrion
Repeat:(A)11
Start:9650 End:9660
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGTAAATCTGAGGCAGGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GATCGAACCCACGACAGACT | |||||||||||||
| Tm_F_1 | 60.066 | Size_F_1 | 20 | Tm_R_1 | 60.12 | Size_R_1 | 20 | PRODUCT Size | 252 bp | Primer Start | 9498 | Primer End | 9749 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATGGTAACCCGTCTCCCTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GATCGAACCCACGACAGACT | |||||||||||||
| Tm_F_2 | 59.691 | Size_F_2 | 20 | Tm_R_2 | 60.12 | Size_R_2 | 20 | PRODUCT Size | 164 bp | Primer Start | 9586 | Primer End | 9749 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGAGAAGACTTCCGTGTTCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAAGCCACCTCACCAATAGC | |||||||||||||
| Tm_F_3 | 59.844 | Size_F_3 | 20 | Tm_R_3 | 59.7 | Size_R_3 | 20 | PRODUCT Size | 206 bp | Primer Start | 9609 | Primer End | 9814 |