Primers Available
Organism:Saccharomyces castellii mitochondrion
Repeat:(ATA)4ttattgtaatt(ATA)4cggatataatattatttataataataaataataaaataaattattt(TA)6(AAAT)3aatcaatataaata(AT)7
Start:325 End:457
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCGAATCCTATATTTCCTACTACCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGAAATGGAAATTGTTGATATTTACTT | |||||||||||||
| Tm_F_1 | 59.053 | Size_F_1 | 25 | Tm_R_1 | 58.5 | Size_R_1 | 27 | PRODUCT Size | 272 bp | Primer Start | 278 | Primer End | 549 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCGAATCCTATATTTCCTACTACCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGAAATGGAAATTGTTGATATTTACT | |||||||||||||
| Tm_F_2 | 59.053 | Size_F_2 | 25 | Tm_R_2 | 57.354 | Size_R_2 | 26 | PRODUCT Size | 272 bp | Primer Start | 278 | Primer End | 549 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTCGAATCCTATATTTCCTACTACCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGAAATGGAAATTGTTGATATTTACTT | |||||||||||||
| Tm_F_3 | 60.123 | Size_F_3 | 26 | Tm_R_3 | 58.5 | Size_R_3 | 27 | PRODUCT Size | 273 bp | Primer Start | 277 | Primer End | 549 |