Primers Available
Organism: Elassoma evergladei mitochondrion
Repeat:(AACC)3
Start:8403 End:8414
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCCTTGGCAGTACCCCTATG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACACCAAGGGCTAAAGGACG | |||||||||||||
| Tm_F_1 | 60.179 | Size_F_1 | 20 | Tm_R_1 | 59.964 | Size_R_1 | 20 | PRODUCT Size | 167 bp | Primer Start | 8354 | Primer End | 8520 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCCTTGGCAGTACCCCTATG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGCTGTTAACCGAACACCA | |||||||||||||
| Tm_F_2 | 60.179 | Size_F_2 | 20 | Tm_R_2 | 59.819 | Size_R_2 | 20 | PRODUCT Size | 181 bp | Primer Start | 8354 | Primer End | 8534 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGCCTTGGCAGTACCCCTAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACACCAAGGGCTAAAGGACG | |||||||||||||
| Tm_F_3 | 60.328 | Size_F_3 | 20 | Tm_R_3 | 59.964 | Size_R_3 | 20 | PRODUCT Size | 168 bp | Primer Start | 8353 | Primer End | 8520 |