Primers Available
Organism: Trachurus japonicus mitochondrion
Repeat:(CTCC)3
Start:5824 End:5835
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATTCCGCTAATGATCGGGGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGAGACCCCTGCTAGGTGAA | |||||||||||||
| Tm_F_1 | 60.322 | Size_F_1 | 20 | Tm_R_1 | 59.884 | Size_R_1 | 20 | PRODUCT Size | 222 bp | Primer Start | 5757 | Primer End | 5978 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATTCCGCTAATGATCGGGGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCCTGCTAGGTGAAGGGAGA | |||||||||||||
| Tm_F_2 | 60.322 | Size_F_2 | 20 | Tm_R_2 | 60.326 | Size_R_2 | 20 | PRODUCT Size | 216 bp | Primer Start | 5757 | Primer End | 5972 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATTCCGCTAATGATCGGGGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATGAGACCCCTGCTAGGTGA | |||||||||||||
| Tm_F_3 | 60.322 | Size_F_3 | 20 | Tm_R_3 | 59.66 | Size_R_3 | 20 | PRODUCT Size | 223 bp | Primer Start | 5757 | Primer End | 5979 |