Primers Available
Organism: Lyciasalamandra atifi mitochondrion
Repeat:(AT)6ctacataattaccactaaacacaaactcaattgagaggactaccacccaattaaccaaaaaccaaaccaacaggagaaccag(ATAC)3
Start:13681 End:13786
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CACCAAAAGACACCCCTCGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTATCCTGAGGCGTGGGGAA | |||||||||||||
| Tm_F_1 | 59.892 | Size_F_1 | 20 | Tm_R_1 | 60.619 | Size_R_1 | 20 | PRODUCT Size | 272 bp | Primer Start | 13554 | Primer End | 13825 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCAAAAGACACCCCTCGAGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGCAGAGCCTTATCCTGAGG | |||||||||||||
| Tm_F_2 | 59.603 | Size_F_2 | 20 | Tm_R_2 | 59.604 | Size_R_2 | 20 | PRODUCT Size | 279 bp | Primer Start | 13556 | Primer End | 13834 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACCAAAAGACACCCCTCGAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGCAGAGCCTTATCCTGAGG | |||||||||||||
| Tm_F_3 | 59.603 | Size_F_3 | 20 | Tm_R_3 | 59.604 | Size_R_3 | 20 | PRODUCT Size | 280 bp | Primer Start | 13555 | Primer End | 13834 |