Primers Available
Organism: Lotus japonicus chloroplast
Repeat:(T)12
Start:33855 End:33866
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATTCGGGACAAATCGCAACC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGATTTGTTCGACTCGATTAGA | |||||||||||||
| Tm_F_1 | 59.191 | Size_F_1 | 20 | Tm_R_1 | 57.422 | Size_R_1 | 23 | PRODUCT Size | 274 bp | Primer Start | 33737 | Primer End | 34010 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCATGCCGCCAAAACGATAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGGTTTACATATACTGACAGTTGCTG | |||||||||||||
| Tm_F_2 | 59.546 | Size_F_2 | 20 | Tm_R_2 | 59.963 | Size_R_2 | 26 | PRODUCT Size | 258 bp | Primer Start | 33659 | Primer End | 33916 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCATGCCGCCAAAACGATAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGGGTTTACATATACTGACAGTTGCT | |||||||||||||
| Tm_F_3 | 59.546 | Size_F_3 | 20 | Tm_R_3 | 60.07 | Size_R_3 | 26 | PRODUCT Size | 259 bp | Primer Start | 33659 | Primer End | 33917 |