Primers Available
Organism:Naegleria gruberi mitochondrion
Repeat:(T)13
Start:34083 End:34095
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CATTTAATGATCGCACATGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GACCTACAATAATTAAAAATTCACCAA | |||||||||||||
| Tm_F_1 | 59.391 | Size_F_1 | 21 | Tm_R_1 | 58.138 | Size_R_1 | 27 | PRODUCT Size | 214 bp | Primer Start | 33954 | Primer End | 34167 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCACATGGATTAGTATCAGGTGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACCAAAGCGAATAAACTGTACATA | |||||||||||||
| Tm_F_2 | 60.626 | Size_F_2 | 23 | Tm_R_2 | 57.126 | Size_R_2 | 24 | PRODUCT Size | 280 bp | Primer Start | 33966 | Primer End | 34245 |
| FORWARD_PRIMER_3 | |||||||||||||
| CATTTAATGATCGCACATGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGACCTACAATAATTAAAAATTCACCA | |||||||||||||
| Tm_F_3 | 59.391 | Size_F_3 | 21 | Tm_R_3 | 57.84 | Size_R_3 | 27 | PRODUCT Size | 215 bp | Primer Start | 33954 | Primer End | 34168 |