Primers Available
Organism:Naegleria gruberi mitochondrion
Repeat:(A)15
Start:31786 End:31800
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTATAATGTTGCTTTTATTCGTTTAGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAAACGCATTGTATTTGCATGT | |||||||||||||
| Tm_F_1 | 57.104 | Size_F_1 | 27 | Tm_R_1 | 59.433 | Size_R_1 | 22 | PRODUCT Size | 220 bp | Primer Start | 31670 | Primer End | 31889 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTATAATGTTGCTTTTATTCGTTTAGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAACGCATTGTATTTGCATGT | |||||||||||||
| Tm_F_2 | 57.104 | Size_F_2 | 27 | Tm_R_2 | 58.11 | Size_R_2 | 21 | PRODUCT Size | 219 bp | Primer Start | 31670 | Primer End | 31888 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTATAATGTTGCTTTTATTCGTTTAGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAAAACGCATTGTATTTGCATGT | |||||||||||||
| Tm_F_3 | 57.104 | Size_F_3 | 27 | Tm_R_3 | 60.629 | Size_R_3 | 23 | PRODUCT Size | 221 bp | Primer Start | 31670 | Primer End | 31890 |