Primers Available
Organism:Naegleria gruberi mitochondrion
Repeat:(A)10ttaaataaaaaa(T)13
Start:45559 End:45593
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTTACCAACTCAATTATTACCATTAGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCTGCCTATAATATTGAAGTAAAGAA | |||||||||||||
| Tm_F_1 | 58.158 | Size_F_1 | 27 | Tm_R_1 | 58.964 | Size_R_1 | 27 | PRODUCT Size | 267 bp | Primer Start | 45471 | Primer End | 45737 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTTACCAACTCAATTATTACCATTAGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGCTGCCTATAATATTGAAGTAAAGA | |||||||||||||
| Tm_F_2 | 58.158 | Size_F_2 | 27 | Tm_R_2 | 57.859 | Size_R_2 | 26 | PRODUCT Size | 267 bp | Primer Start | 45471 | Primer End | 45737 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTACCAACTCAATTATTACCATTAGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGCTGCCTATAATATTGAAGTAAAGAA | |||||||||||||
| Tm_F_3 | 57.024 | Size_F_3 | 26 | Tm_R_3 | 58.964 | Size_R_3 | 27 | PRODUCT Size | 266 bp | Primer Start | 45472 | Primer End | 45737 |