Primers Available
Organism:Physarum polycephalum mitochondrion
Repeat:(TTTA)3
Start:31675 End:31686
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCATTGCAAATACTGCGTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TACTCCTGCAAAACCAAGGC | |||||||||||||
| Tm_F_1 | 58.846 | Size_F_1 | 20 | Tm_R_1 | 60.249 | Size_R_1 | 20 | PRODUCT Size | 237 bp | Primer Start | 31605 | Primer End | 31841 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCATTGCAAATACTGCGTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGTACTCCTGCAAAACCAA | |||||||||||||
| Tm_F_2 | 58.846 | Size_F_2 | 20 | Tm_R_2 | 59.17 | Size_R_2 | 20 | PRODUCT Size | 240 bp | Primer Start | 31605 | Primer End | 31844 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGCATTGCAAATACTGCGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TACTCCTGCAAAACCAAGGC | |||||||||||||
| Tm_F_3 | 60.822 | Size_F_3 | 19 | Tm_R_3 | 60.249 | Size_R_3 | 20 | PRODUCT Size | 238 bp | Primer Start | 31604 | Primer End | 31841 |