Primers Available
Organism: Chlorella vulgaris chloroplast
Repeat:(T)12
Start:355 End:366
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCGCCAGGAAGAAGTTGTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGAACGAAAAACTCATTGAATCA | |||||||||||||
| Tm_F_1 | 59.331 | Size_F_1 | 20 | Tm_R_1 | 57.367 | Size_R_1 | 24 | PRODUCT Size | 280 bp | Primer Start | 179 | Primer End | 458 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCTGCGCCAGGAAGAAGTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGAGAAAAAGAAAAGAAGCCTTTCT | |||||||||||||
| Tm_F_2 | 59.891 | Size_F_2 | 20 | Tm_R_2 | 57.59 | Size_R_2 | 25 | PRODUCT Size | 235 bp | Primer Start | 175 | Primer End | 409 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTCTGCGCCAGGAAGAAGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGAGAAAAAGAAAAGAAGCCTTTCT | |||||||||||||
| Tm_F_3 | 59.891 | Size_F_3 | 20 | Tm_R_3 | 57.59 | Size_R_3 | 25 | PRODUCT Size | 236 bp | Primer Start | 174 | Primer End | 409 |