Primers Available
Organism:Acanthamoeba castellanii mitochondrion
Repeat:(T)10
Start:9112 End:9121
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAGGATCCTACAACGCCAAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACGTTTTGATTCGGGAAGAT | |||||||||||||
| Tm_F_1 | 59.955 | Size_F_1 | 20 | Tm_R_1 | 58.504 | Size_R_1 | 20 | PRODUCT Size | 162 bp | Primer Start | 9056 | Primer End | 9217 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTTCAGGATCCTACAACGCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACGTTTTGATTCGGGAAGAT | |||||||||||||
| Tm_F_2 | 60.074 | Size_F_2 | 20 | Tm_R_2 | 58.504 | Size_R_2 | 20 | PRODUCT Size | 165 bp | Primer Start | 9053 | Primer End | 9217 |
| FORWARD_PRIMER_3 | |||||||||||||
| CAGGATCCTACAACGCCAAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TACGTTTTGATTCGGGAAGA | |||||||||||||
| Tm_F_3 | 59.955 | Size_F_3 | 20 | Tm_R_3 | 58.214 | Size_R_3 | 20 | PRODUCT Size | 163 bp | Primer Start | 9056 | Primer End | 9218 |