Primers Available
Organism:Acanthamoeba castellanii mitochondrion
Repeat:(T)10
Start:23446 End:23455
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCGTTATCGGTTGCTAATAAGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCCGGAACATTATTAATTGGG | |||||||||||||
| Tm_F_1 | 60.012 | Size_F_1 | 22 | Tm_R_1 | 60.385 | Size_R_1 | 21 | PRODUCT Size | 146 bp | Primer Start | 23378 | Primer End | 23523 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGCTGTTTTACACGAAAGTGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCCGGAACATTATTAATTGGG | |||||||||||||
| Tm_F_2 | 58.881 | Size_F_2 | 21 | Tm_R_2 | 60.385 | Size_R_2 | 21 | PRODUCT Size | 189 bp | Primer Start | 23335 | Primer End | 23523 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGCTGTTTTACACGAAAGTGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCCGGAACATTATTAATTGGG | |||||||||||||
| Tm_F_3 | 60.2 | Size_F_3 | 22 | Tm_R_3 | 60.385 | Size_R_3 | 21 | PRODUCT Size | 190 bp | Primer Start | 23334 | Primer End | 23523 |