Primers Available
Organism:Acanthamoeba castellanii mitochondrion
Repeat:(A)14
Start:35726 End:35739
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCGCTCTAAATTGTCCAAGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCCGAAACAGTTTTTAACG | |||||||||||||
| Tm_F_1 | 59.348 | Size_F_1 | 21 | Tm_R_1 | 58.863 | Size_R_1 | 20 | PRODUCT Size | 266 bp | Primer Start | 35505 | Primer End | 35770 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCGCTCTAAATTGTCCAAGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGACAGATGCCGAAACAGT | |||||||||||||
| Tm_F_2 | 59.348 | Size_F_2 | 21 | Tm_R_2 | 59.846 | Size_R_2 | 19 | PRODUCT Size | 273 bp | Primer Start | 35505 | Primer End | 35777 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCGCTCTAAATTGTCCAAGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGCCGAAACAGTTTTTAACG | |||||||||||||
| Tm_F_3 | 57.972 | Size_F_3 | 20 | Tm_R_3 | 58.863 | Size_R_3 | 20 | PRODUCT Size | 265 bp | Primer Start | 35506 | Primer End | 35770 |