Primers Available
Organism: Pinus thunbergii chloroplast
Repeat:(TTTA)3
Start:41453 End:41464
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACTTCCGTTACAAGGAACTCGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCCGAACCTAAAGCGTACA | |||||||||||||
| Tm_F_1 | 59.375 | Size_F_1 | 22 | Tm_R_1 | 59.682 | Size_R_1 | 20 | PRODUCT Size | 277 bp | Primer Start | 41383 | Primer End | 41659 |
| FORWARD_PRIMER_2 | |||||||||||||
| CACTTCCGTTACAAGGAACTCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGCCGAACCTAAAGCGTACA | |||||||||||||
| Tm_F_2 | 59.27 | Size_F_2 | 22 | Tm_R_2 | 59.682 | Size_R_2 | 20 | PRODUCT Size | 278 bp | Primer Start | 41382 | Primer End | 41659 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACTTCCGTTACAAGGAACTCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGCCGAACCTAAAGCGTACA | |||||||||||||
| Tm_F_3 | 57.977 | Size_F_3 | 21 | Tm_R_3 | 59.682 | Size_R_3 | 20 | PRODUCT Size | 277 bp | Primer Start | 41383 | Primer End | 41659 |