Primers Available
Organism: Pinus thunbergii chloroplast
Repeat:(TCTA)3
Start:51050 End:51061
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTCAATGGGATTGTGGCGTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GATGGTTGTATCGGGCCCTT | |||||||||||||
| Tm_F_1 | 59.829 | Size_F_1 | 20 | Tm_R_1 | 59.817 | Size_R_1 | 20 | PRODUCT Size | 150 bp | Primer Start | 50937 | Primer End | 51086 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCAATGGGATTGTGGCGTGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GATGGTTGTATCGGGCCCTT | |||||||||||||
| Tm_F_2 | 60.251 | Size_F_2 | 20 | Tm_R_2 | 59.817 | Size_R_2 | 20 | PRODUCT Size | 149 bp | Primer Start | 50938 | Primer End | 51086 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTCAATGGGATTGTGGCGTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGCAGCTCACTGTTCTGACT | |||||||||||||
| Tm_F_3 | 59.829 | Size_F_3 | 20 | Tm_R_3 | 60.319 | Size_R_3 | 20 | PRODUCT Size | 172 bp | Primer Start | 50937 | Primer End | 51108 |