Primers Available
Organism: Pinus thunbergii chloroplast
Repeat:(AAAG)3
Start:110312 End:110323
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACGGGTTCAAGTCAAATAGCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAGATCTCCCTCCAAACCGT | |||||||||||||
| Tm_F_1 | 57.865 | Size_F_1 | 21 | Tm_R_1 | 58.342 | Size_R_1 | 20 | PRODUCT Size | 254 bp | Primer Start | 110200 | Primer End | 110453 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACGGGTTCAAGTCAAATAGCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTCGAATACGGCTTTCCACA | |||||||||||||
| Tm_F_2 | 57.865 | Size_F_2 | 21 | Tm_R_2 | 58.283 | Size_R_2 | 20 | PRODUCT Size | 221 bp | Primer Start | 110200 | Primer End | 110420 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACGGGTTCAAGTCAAATAGCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GATCTCCCTCCAAACCGTACA | |||||||||||||
| Tm_F_3 | 57.865 | Size_F_3 | 21 | Tm_R_3 | 59.171 | Size_R_3 | 21 | PRODUCT Size | 252 bp | Primer Start | 110200 | Primer End | 110451 |