Primers Available
Organism: Pinus thunbergii chloroplast
Repeat:(A)12
Start:79987 End:79998
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTCCGGTCTTGAAAACCGGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATCGGCGGGATAGGAGTCTT | |||||||||||||
| Tm_F_1 | 60.25 | Size_F_1 | 20 | Tm_R_1 | 60.179 | Size_R_1 | 20 | PRODUCT Size | 191 bp | Primer Start | 79889 | Primer End | 80079 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTGGTTAATGGCTCCGGTCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATCGGCGGGATAGGAGTCTT | |||||||||||||
| Tm_F_2 | 59.748 | Size_F_2 | 20 | Tm_R_2 | 60.179 | Size_R_2 | 20 | PRODUCT Size | 202 bp | Primer Start | 79878 | Primer End | 80079 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGTGGTTAATGGCTCCGGTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATCGGCGGGATAGGAGTCTT | |||||||||||||
| Tm_F_3 | 59.748 | Size_F_3 | 20 | Tm_R_3 | 60.179 | Size_R_3 | 20 | PRODUCT Size | 203 bp | Primer Start | 79877 | Primer End | 80079 |