Primers Available
Organism:Prototheca wickerhamii mitochondrion
Repeat:(TTAT)3
Start:1559 End:1570
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCATGTTTCCAGAGTCACA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGCTAAAATAAGCAATGTTTTAATGC | |||||||||||||
| Tm_F_1 | 59.52 | Size_F_1 | 20 | Tm_R_1 | 59.966 | Size_R_1 | 26 | PRODUCT Size | 276 bp | Primer Start | 1393 | Primer End | 1668 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGGGATTTGAACCCATGTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCAATGTTTTAATGCATTTACTAGC | |||||||||||||
| Tm_F_2 | 60.029 | Size_F_2 | 20 | Tm_R_2 | 58.48 | Size_R_2 | 25 | PRODUCT Size | 276 bp | Primer Start | 1382 | Primer End | 1657 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGGATTTGAACCCATGTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGCAATGTTTTAATGCATTTACTAGC | |||||||||||||
| Tm_F_3 | 60.029 | Size_F_3 | 20 | Tm_R_3 | 59.28 | Size_R_3 | 26 | PRODUCT Size | 277 bp | Primer Start | 1382 | Primer End | 1658 |