Primers Available
Organism:Prototheca wickerhamii mitochondrion
Repeat:(TA)12attgatatgaataatttatatatatttacttttgtttttaagaaagtatatt(TTAA)3
Start:21514 End:21601
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAATGGGCAAAAAGGGACTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTCATAATGGTTGCCGTCCT | |||||||||||||
| Tm_F_1 | 59.814 | Size_F_1 | 20 | Tm_R_1 | 59.955 | Size_R_1 | 20 | PRODUCT Size | 231 bp | Primer Start | 21407 | Primer End | 21637 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAAAGGGACTTGAACCCCTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTCATAATGGTTGCCGTCCT | |||||||||||||
| Tm_F_2 | 60.332 | Size_F_2 | 20 | Tm_R_2 | 59.955 | Size_R_2 | 20 | PRODUCT Size | 222 bp | Primer Start | 21416 | Primer End | 21637 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAGGGACTTGAACCCCTGAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTCATAATGGTTGCCGTCCT | |||||||||||||
| Tm_F_3 | 60.349 | Size_F_3 | 20 | Tm_R_3 | 59.955 | Size_R_3 | 20 | PRODUCT Size | 220 bp | Primer Start | 21418 | Primer End | 21637 |