Primers Available
Organism:Prototheca wickerhamii mitochondrion
Repeat:(ATA)4
Start:7370 End:7381
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AATTGGATCTCCACCACCTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATTTAATATGCGCGCTCCAG | |||||||||||||
| Tm_F_1 | 59.779 | Size_F_1 | 20 | Tm_R_1 | 60.213 | Size_R_1 | 20 | PRODUCT Size | 184 bp | Primer Start | 7274 | Primer End | 7457 |
| FORWARD_PRIMER_2 | |||||||||||||
| CACCTGCAGGGTCAAAGAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TATTGCAAGTCACTCTGGCG | |||||||||||||
| Tm_F_2 | 60.822 | Size_F_2 | 20 | Tm_R_2 | 60.011 | Size_R_2 | 20 | PRODUCT Size | 272 bp | Primer Start | 7288 | Primer End | 7559 |
| FORWARD_PRIMER_3 | |||||||||||||
| AATTGGATCTCCACCACCTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TATTTAATATGCGCGCTCCA | |||||||||||||
| Tm_F_3 | 59.779 | Size_F_3 | 20 | Tm_R_3 | 59.316 | Size_R_3 | 20 | PRODUCT Size | 185 bp | Primer Start | 7274 | Primer End | 7458 |