Primers Available
Organism:Prototheca wickerhamii mitochondrion
Repeat:(AATA)3
Start:22527 End:22538
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAATTTGTGCACATGCTTGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGACAAAAACAGGAAAACGTAAA | |||||||||||||
| Tm_F_1 | 57.761 | Size_F_1 | 20 | Tm_R_1 | 58.752 | Size_R_1 | 23 | PRODUCT Size | 270 bp | Primer Start | 22347 | Primer End | 22616 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAATTTGTGCACATGCTTGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTGACAAAAACAGGAAAACGTAAA | |||||||||||||
| Tm_F_2 | 57.761 | Size_F_2 | 20 | Tm_R_2 | 59.51 | Size_R_2 | 24 | PRODUCT Size | 271 bp | Primer Start | 22347 | Primer End | 22617 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCTAAGCCGTAAATTTGTGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGACAAAAACAGGAAAACGTAAA | |||||||||||||
| Tm_F_3 | 57.486 | Size_F_3 | 20 | Tm_R_3 | 58.752 | Size_R_3 | 23 | PRODUCT Size | 280 bp | Primer Start | 22337 | Primer End | 22616 |