Primers Available
Organism:Prototheca wickerhamii mitochondrion
Repeat:(AAAT)3
Start:41285 End:41296
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCTCGTCAAAAACGTGATGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAAAATTGATTTCAAAAAGTGGTT | |||||||||||||
| Tm_F_1 | 59.847 | Size_F_1 | 20 | Tm_R_1 | 57.401 | Size_R_1 | 24 | PRODUCT Size | 277 bp | Primer Start | 41193 | Primer End | 41469 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGAATTACATAGCGCAGCCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAAAATTGATTTCAAAAAGTGGTT | |||||||||||||
| Tm_F_2 | 60.378 | Size_F_2 | 20 | Tm_R_2 | 57.401 | Size_R_2 | 24 | PRODUCT Size | 260 bp | Primer Start | 41210 | Primer End | 41469 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCTCGTCAAAAACGTGATGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAAAATTGATTTCAAAAAGTGGTTA | |||||||||||||
| Tm_F_3 | 59.847 | Size_F_3 | 20 | Tm_R_3 | 57.49 | Size_R_3 | 25 | PRODUCT Size | 277 bp | Primer Start | 41193 | Primer End | 41469 |