Primers Available
Organism:Podospora anserina mitochondrion
Repeat:(CTGG)4
Start:140 End:155
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTAATATACGGGCTGACGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGCCAGCCAGCCTACTAAAC | |||||||||||||
| Tm_F_1 | 59.81 | Size_F_1 | 20 | Tm_R_1 | 61.136 | Size_R_1 | 20 | PRODUCT Size | 261 bp | Primer Start | 115 | Primer End | 375 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTAATATACGGGCTGACGGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCCGCGTCTATGTATGTAGG | |||||||||||||
| Tm_F_2 | 59.81 | Size_F_2 | 20 | Tm_R_2 | 58.294 | Size_R_2 | 20 | PRODUCT Size | 279 bp | Primer Start | 115 | Primer End | 393 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCAGTTATCCAGGGTAGAGGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTGTGTGAGGAGGAGCTTG | |||||||||||||
| Tm_F_3 | 59.828 | Size_F_3 | 21 | Tm_R_3 | 59.007 | Size_R_3 | 20 | PRODUCT Size | 184 bp | Primer Start | 12 | Primer End | 195 |