Primers Available
Organism:Paramecium aurelia mitochondrion
Repeat:(ATA)4
Start:627 End:638
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTCTGCCGCTTTAACCACTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACTAGCCTCTGGTTCGGGAT | |||||||||||||
| Tm_F_1 | 59.882 | Size_F_1 | 20 | Tm_R_1 | 60.096 | Size_R_1 | 20 | PRODUCT Size | 268 bp | Primer Start | 592 | Primer End | 859 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGCTTTAACCACTCAGCCAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACTAGCCTCTGGTTCGGGAT | |||||||||||||
| Tm_F_2 | 60.27 | Size_F_2 | 20 | Tm_R_2 | 60.096 | Size_R_2 | 20 | PRODUCT Size | 262 bp | Primer Start | 598 | Primer End | 859 |
| FORWARD_PRIMER_3 | |||||||||||||
| CAGTCTGCCGCTTTAACCAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACTAGCCTCTGGTTCGGGAT | |||||||||||||
| Tm_F_3 | 60.827 | Size_F_3 | 20 | Tm_R_3 | 60.096 | Size_R_3 | 20 | PRODUCT Size | 270 bp | Primer Start | 590 | Primer End | 859 |