Primers Available
Organism: Nephroselmis olivacea chloroplast
Repeat:(AGA)4
Start:74678 End:74689
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGCGTTCCACGTCTAGGAAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAAGGGAATTTCGCACGAGC | |||||||||||||
| Tm_F_1 | 60.179 | Size_F_1 | 20 | Tm_R_1 | 59.903 | Size_R_1 | 20 | PRODUCT Size | 152 bp | Primer Start | 74604 | Primer End | 74755 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGCGTTCCACGTCTAGGAAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGACGCATGATTGCTTCACC | |||||||||||||
| Tm_F_2 | 60.179 | Size_F_2 | 20 | Tm_R_2 | 59.901 | Size_R_2 | 20 | PRODUCT Size | 191 bp | Primer Start | 74604 | Primer End | 74794 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGCGTTCCACGTCTAGGAAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGATAGCCCCCAAACACTCC | |||||||||||||
| Tm_F_3 | 60.179 | Size_F_3 | 20 | Tm_R_3 | 60.107 | Size_R_3 | 20 | PRODUCT Size | 263 bp | Primer Start | 74604 | Primer End | 74866 |