Primers Available
Organism: Nephroselmis olivacea chloroplast
Repeat:(AAAG)3
Start:50392 End:50403
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CACGAGGCAAGGAGACAGAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGTTCAATCCCAACGCCTTG | |||||||||||||
| Tm_F_1 | 59.682 | Size_F_1 | 20 | Tm_R_1 | 60.11 | Size_R_1 | 20 | PRODUCT Size | 278 bp | Primer Start | 50360 | Primer End | 50637 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGAAAAGACCCACGAGGCAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGCCTTGAAACGTTTTGCGA | |||||||||||||
| Tm_F_2 | 59.818 | Size_F_2 | 20 | Tm_R_2 | 60.59 | Size_R_2 | 20 | PRODUCT Size | 275 bp | Primer Start | 50350 | Primer End | 50624 |
| FORWARD_PRIMER_3 | |||||||||||||
| CACGAGGCAAGGAGACAGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATCCCAACGCCTTGAAACGT | |||||||||||||
| Tm_F_3 | 59.682 | Size_F_3 | 20 | Tm_R_3 | 60.536 | Size_R_3 | 20 | PRODUCT Size | 272 bp | Primer Start | 50360 | Primer End | 50631 |