Primers Available
Organism: Plestiodon egregius mitochondrion
Repeat:(CCAAA)3
Start:13920 End:13934
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AATTTCCCCAGGTTTCCGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCCGTATTATGGGGCAGCAG | |||||||||||||
| Tm_F_1 | 60.252 | Size_F_1 | 20 | Tm_R_1 | 59.604 | Size_R_1 | 20 | PRODUCT Size | 182 bp | Primer Start | 13806 | Primer End | 13987 |
| FORWARD_PRIMER_2 | |||||||||||||
| AATTTCCCCAGGTTTCCGGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTCCGTATTATGGGGCAGCA | |||||||||||||
| Tm_F_2 | 60.252 | Size_F_2 | 20 | Tm_R_2 | 59.604 | Size_R_2 | 20 | PRODUCT Size | 183 bp | Primer Start | 13806 | Primer End | 13988 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCACCCCCTCTACACCAAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGCAGCAGGGTTAGTGGTTT | |||||||||||||
| Tm_F_3 | 59.88 | Size_F_3 | 20 | Tm_R_3 | 60.54 | Size_R_3 | 20 | PRODUCT Size | 276 bp | Primer Start | 13700 | Primer End | 13975 |