Primers Available
Organism: Plestiodon egregius mitochondrion
Repeat:(AC)6
Start:16933 End:16944
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTGACGATTCCCCTGCAACT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTAGGTGGGTTACGGGTGTG | |||||||||||||
| Tm_F_1 | 60.036 | Size_F_1 | 20 | Tm_R_1 | 60.037 | Size_R_1 | 20 | PRODUCT Size | 274 bp | Primer Start | 16699 | Primer End | 16972 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCGGCAGTACGTGATTTCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTAGGTGGGTTACGGGTGTG | |||||||||||||
| Tm_F_2 | 60.108 | Size_F_2 | 20 | Tm_R_2 | 60.037 | Size_R_2 | 20 | PRODUCT Size | 223 bp | Primer Start | 16750 | Primer End | 16972 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAAATTGCCGCGACAAACGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTAGGTGGGTTACGGGTGTG | |||||||||||||
| Tm_F_3 | 60.249 | Size_F_3 | 20 | Tm_R_3 | 60.037 | Size_R_3 | 20 | PRODUCT Size | 248 bp | Primer Start | 16725 | Primer End | 16972 |